ANG II inhibits insulin-mediated production of PI 3,4,5-trisphosphates via a Ca -dependent but PKC-independent pathway in the cardiomyocytes

Ikushima M, Ishii M, Ohishi M, Yamamoto K, Ogihara T, Rakugi H, Kurachi Y. ANG II inhibits insulin-mediated production of PI 3,4,5-trisphosphates via a Ca -dependent but PKCindependent pathway in the cardiomyocytes. Am J Physiol Heart Circ Physiol 299: H680 –H689, 2010. First published July 2, 2010; doi:10.1152/ajpheart.00220.2009.—Insulin resistance (IR) is a condition where different organs are refractory to insulin stimulation of glucose uptake. ANG II has been suggested to be involved in the development of IR in the heart. The precise mechanism by which this occurs is still unknown. Here we have used dynamic fluorescent imaging techniques to show that ANG II inhibits insulin production of phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] in cardiac myocytes. Fluorophore (Venus)-conjugated cAMP-dependent protein kinase-pleckstrin homology domain, which specifically binds to PI(3,4,5)P3, was transfected in neonatal rat cardiac myocytes. Insulin induced a robust increase in the fluorescence intensity at the cell surface, which was diminished by application of ANG II. The inhibitory action of ANG II was antagonized by RNH-6270 (an angiotensin type 1 receptor antagonist) but not by PD-122370 (an angiotensin type 2 receptor antagonist). BAPTA-AM (Ca chelator) largely attenuated the ANG II effect, whereas K-252b (PKC inhibitor) did not. Furthermore, an elevation of intracellular Ca induced by ionomycin mimicked the ANG II effect. Therefore, it is suggested that ANG II antagonizes insulin-mediated production of PI(3,4,5)P3 via a Ca dependent but PKC-independent pathway in cardiac myocytes.